Journal: Advanced Science

Article Title: Phosphomevalonate Kinase Controls β ‐Catenin Signaling via the Metabolite 5‐Diphosphomevalonate

doi: 10.1002/advs.202204909

Figure Lengend Snippet: PMVK expression is increased in human HCC and correlates with poor survival. A) Western blot analysis for the indicated proteins in human HCCs. T, tumor; N, adjacent normal tissue. B–F) The intensities of PMVK, β ‐catenin (total and p‐S184), c‐Myc and Cyclin D1 for (A) were quantified by densitometry, with β ‐actin as a normalizer. Data are shown as mean ± SD. G–O) Correlation of different protein levels in HCC tissues from (A). Each point is an individual sample. P) PMVK mRNA levels in normal liver and HCCs from TCGA. Q) PMVK gene copy number in normal liver and tumor tissues from TCGA. R) Correlation of PMVK mRNA level with its DNA copy number in HCC tissues from TCGA dataset. Each point is an individual sample. S) Kaplan–Meier curves with univariate analysis of the survival of patients with HCC based on high versus low expression of PMVK. Data are shown as mean ± SD. The P values were determined by paired two‐sided Student's t ‐test for (B–F) and unpaired two‐sided Student's t ‐test for (P) and (Q). The correlation coefficient ( r ) and p values in (G–O and R) were determined using two‐tailed Pearson correlation analysis.

Article Snippet: The correlation coefficient ( r ) and p values were obtained from Pearson correlation analysis, Pearson's r was calculated by GraphPad Prism 8.

Techniques: Expressing, Western Blot, Two Tailed Test